The SARS-CoV-2 detection kit (RT-PCR) is a real time reverse transcription followed by polymerase chain reaction (rRT-PCR) test. The SARS-CoV-2 primer and probe sets are designed to detect RNA from SARS-CoV-2 in respiratory specimens from patients who are suspected of SARS-CoV-2 infection by their healthcare provider. This kit is used for qualitative detection of the ORF1ab gene from SARS-CoV-2, and T478K, P681R mutations of the SARS-CoV-2 B.1. 617.variants. P681R mutation is present in all sublineages of B.1.617 (B.1.617.1, B.1.617.2 and B.1.617.3). T478K is specific for B.1.617.2 (Delta) variant

After PCR reaction, fluorescent signal from target is acquired and further analyzed by a real time PCR instrument. As a result, genes specific to novel coronavirus SARS-CoV-2 and B.1.617 lineage can be detected with high precision.

In particular, ORF1ab probe contains FAM label, T478K probe contains HEX/Joe label, P681R probe contains ROX label, Internal control probe contains Cy5 label.

Ref No. 02.01.1050

Kit Components

• SARS-CoV-2 RT-PCR Detection Buffer

• Enzyme Mix

• SARS-CoV-2 Positive Control

• SARS-CoV-2 Negative Control