Novel Coronavirus (2019-nCoV) Real Time Multiplex RT-PCR Kit (Detection for 3 Genes) is used for the in vitro qualitative detection of 2019 novel coronavirus (2019-nCoV) RNA in

nasopharyngeal swab, oropharyngeal swab and sputum specimens by real time PCR systems. It is considered as an aid in the diagnosis of the 2019-nCoV infection.

The principle of the real-time detection is based on the fluorogenic 5’nuclease assay. During the PCR reaction, the DNA polymerase cleaves the probe at the 5’ end and separates the reporter dye from the quencher dye only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent signal generated by the cleaved reporter dye, which is monitored real-time by the PCR detection system. The PCR cycle at which an increase in the fluorescence signal is detected initially (Ct) is proportional to the amount of the specific PCR product. Monitoring the fluorescence intensities in real time allows the detection of the accumulating product without having to re-open the reaction tube after the amplification.

Real time reverse-transcription polymerase chain reaction (real-time RT-PCR) is used when the starting material is RNA. In this method, RNA is first transcribed into the complementary DNA (cDNA) by reverse transcriptase from total RNA. The cDNA is then used as a template for the real time PCR.

Ref No. RR-0479-02

Kit Content:

• Novel CoV (2019-nCoV) Super Mix

• RT-PCR Enzyme Mix Novel CoV (2019-nCoV)

• Internal Control Novel CoV (2019-nCoV)

• Negative Control Novel CoV

• Positive Control